![]() 125000003729 nucleotide group Chemical group 0.000 claims description 20.239000007795 chemical reaction product Substances 0.000 claims description 22.238000006243 chemical reaction Methods 0.000 claims description 58.230000004544 DNA amplification Effects 0.000 claims abstract description 7.238000002844 melting Methods 0.000 claims abstract description 25.239000011541 reaction mixture Substances 0.000 claims abstract description 39.LYCAIKOWRPUZTN-UHFFFAOYSA-N glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 42.239000000203 mixture Substances 0.000 claims abstract description 46.PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 261.229920003013 deoxyribonucleic acid Polymers 0.000 title claims abstract description 131.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority claimed from US09/878,131 external-priority Priority claimed from US09/878,131 external-priority patent/US20030087237A1/en Application filed by Individual filed Critical Individual Publication of CA2449560A1 publication Critical patent/CA2449560A1/en Status Abandoned legal-status Critical Current Links Original Assignee Individual Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Inventor Guo Fan Hong Yongjie Yang Jia Zhu Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Abandoned Application number CA002449560A Other languages French ( fr) Google Patents Low-temperature cycle extension of dna with high priming specificityÄownload PDF Info Publication number CA2449560A1 CA2449560A1 CA002449560A CA2449560A CA2449560A1 CA 2449560 A1 CA2449560 A1 CA 2449560A1 CA 002449560 A CA002449560 A CA 002449560A CA 2449560 A CA2449560 A CA 2449560A CA 2449560 A1 CA2449560 A1 CA 2449560A1 Authority CA Canada Prior art keywords dna primer bacillus dna polymerase cycle Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CA2449560A1 - Low-temperature cycle extension of dna with high priming specificity CA2449560A1 - Low-temperature cycle extension of dna with high priming specificity ![]()
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